Enzyme immobilization on gold/Fe-oxide composite nanoparticles using a methionine tag

• Enzymes were immobilized on magnetic nanoparticles by a methionine-tag system.
• Methionine-tagged immobilized enzymes showed 98% residual specific activity.
• The system provided orientational immobilization via the formation of Au-S bonds.

We developed a novel technique for immobilizing enzymes on magnetic nanoparticle surfaces by using a methionine-tag system. Au/Fe-oxide composite nanoparticles synthesized through a radiochemical process were used as magnetic nanocarriers. The C-terminus of Tk-subtilisin, a model enzyme, was modified with a methionine tag and mixed with Au/Fe-oxide composite nanoparticles for immobilization via Au-S bonding. Methionine-tagged immobilized enzymes showed 98% residual specific activity, while the untagged enzymes showed 78%. The methionine-tagged immobilized enzymes retained their activities in a wide temperature range of 30–70 °C. Thus, the methionine-tag system provided orientational immobilization via the formation of Au-S bonds, which resulted in structural stability of the immobilized enzymes.

Figure optionsDownload as PowerPoint slide