Factorial design as tool in chitosan nanoparticles development by ionic gelation technique

- This work shows how to obtain chitosan nanoparticles with properties desired.
- The formulation was optimized using a 23 factorial design.
- Nanoparticles were obtained with size <90 nm.
- Nanoparticles were characterized by FTIR, TGA, EDX and TEM analysis.
- Sonication is an important step, otherwise, the nanoparticles agglomerate again.