کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
594730 | 1453985 | 2011 | 7 صفحه PDF | دانلود رایگان |
In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation.
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► Manganese ferrite nanoparticles conjugated to an anti Sticholysin monoclonal antibody.
► Peroxidase-like activity of manganese ferrite nanoparticles.
► Bioanalytical applications of manganese ferrite nanoparticles.
► Immunoassay and protocol for protein separation using manganese ferrite nanoparticles.
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Volume 387, Issues 1–3, 20 August 2011, Pages 118–124