کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5948314 1172378 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mutations in the SORT1 gene are unlikely to cause autosomal dominant hypercholesterolemia
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Mutations in the SORT1 gene are unlikely to cause autosomal dominant hypercholesterolemia
چکیده انگلیسی

ObjectiveTo study whether mutations in the SORT1 gene could be a cause of autosomal dominant hypercholesterolemia and to study the effect of sortilin on the binding and internalization of low density lipoprotein (LDL).Methods842 unrelated hypercholesterolemic subjects without mutations in genes known to cause autosomal dominant hypercholesterolemia, were screened for mutations in the SORT1 gene by DNA sequencing. Transfections of wild-type or mutant SORT1 plasmids in HeLa T-REx cells and the use of siRNA were used to study the effect of sortilin on the number of cell-surface LDL receptors and on the binding and internalization of LDL.ResultsA total of 45 mutations in the SORT1 gene were identified of which 15 were missense mutations. Eight of these were selected for in vitro studies, of which none had a major impact on the amount of LDL bound to the cell surface. There was a positive correlation between the amount of sortilin on the cell surface and the amount of LDL bound. The observation that a mutant sortilin which is predominantly found on the cell surface rather than in post-Golgi compartments, bound very high amounts of LDL, indicates that sortilin does not increase the binding of LDL through an intracellular mechanism. Rather, our data indicate that sortilin binds LDL on the cell surface.ConclusionEven though sortilin binds and internalizes LDL by receptor-mediated endocytosis, mutations in the SORT1 gene are unlikely to cause autosomal dominant hypercholesterolemia and may only have a marginal effect on plasma LDL cholesterol levels.

► 45 mutations in the SORT1 gene have been identified in hypercholesterolemic subjects. ► 8 missense mutations in the SORT1 gene were subjected to in vitro testing. ► Sortilin on the cell surface binds LDL. ► Sortilin does not affect the binding of LDL by an intracellular mechanism. ► Mutations in the SORT1 gene are unlikely to cause severe hypercholesterolemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Atherosclerosis - Volume 225, Issue 2, December 2012, Pages 370-375
نویسندگان
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