کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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594908 | 1453993 | 2011 | 8 صفحه PDF | دانلود رایگان |
An immunoassay based on the enrichment of antibodies by magnetic beads (MB) and their subsequent detection of antigens in a microfluidic chip (MFC)–quartz crystal microbalance (QCM) was developed and investigated. The MB conjugated to goat anti-human-immunoglobulin G (MB-anti-h-IgG) were concentrated within microchannels by the application of a magnetic field, which also functioned as a separation switch outside the MFC. The conjugation of the MB and the anti-h-IgG antibody in the MFC was completed in 1 min. Removal of the magnetic field allowed the resulting tributary buffer solution to flush the MB-anti-h-IgG out of the MFC and onto a QCM chip. The combination of the MB-anti-h-IgG and the QCM chip amplified the detection sensitivity of antigen(s) to 10−1 ng/ml. By controlling the enrichment velocity and sample injection mode in the MFC, a linear response ranging from 10−1 to 103 ng/ml was obtained. This technique establishes a simple, rapid MFC method for the amplification of a QCM-based immunoassay.
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Volume 379, Issues 1–3, 20 April 2011, Pages 2–9