Measuring protein mobility using modern microelectrophoresis

The measurement of the electrophoretic mobility of proteins by microelectrophoresis using up to date, commercially available, instrumentation is discussed. The primary systematic uncertainties and their effects such as joule heating and convection, the presence of aggregates and polarisation concentration are discussed along with methods for their detection, quantification and reduction. Simple measurement strategies are found and excellent agreement between microelectrophoresis and literature values from other techniques is demonstrated.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Thorough uncertainty analysis of protein mobility measurement using microelectrophoresis. ▶ Techniques to reduce primary uncertainties to negligible levels presented. ▶ Precise and accurate measurement of only the mobility of only the protein shown. ▶ Results in excellent agreement with other techniques demonstrated.