کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
597987 1454078 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gold-aggregated, dye-embedded, polymer-protected nanoparticles (GDPNs): A new type of tags for detection with SERS
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Gold-aggregated, dye-embedded, polymer-protected nanoparticles (GDPNs): A new type of tags for detection with SERS
چکیده انگلیسی

Gold-aggregated, dye-embedded, polymer-protected nanoparticles (GDPNs) are unusual aggregates that involve charge-driven aggregation of gold nanoparticles/poly(l-lysine) functionalized with Raman dye and a poly(l-lysine) (PLL) outer layer. Discrete, submicrometer-sized spherical aggregates are formed through three-step layer-by-layer assembly of polymer/nanoparticle. Unlike most of traditional Raman tags, aggregative gold nanoparticles (Au NPs) are used in GDPNs as the active substrate for increasing the signal of surface enhanced Raman scattering (SERS). Furthermore, the outer PLL layer could lock in Au NPs modified with rhodamine B isothiocyanate (RBITC) and lock out external dye molecules. Specifically, PLL as the outer layer exposes an amino-group rich surface to the surrounding environment that should facilitate the conjugation of GDPNs with biomolecules (antibody/antigen, nucleic acid). These advantages suggest the potential of using GDPNs as Raman tags for multiplex and ultrasensitive detection of biomolecules. The Raman tags were characterized using transmission electron microscopy (TEM), UV–vis absorption spectrometry, and Raman spectrometry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Volume 294, Issues 1–3, 15 February 2007, Pages 80–85
نویسندگان
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