کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
598279 1454087 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single-molecule interaction between circularly permuted green fluorescent protein and trypsin by total internal reflection fluorescence microscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Single-molecule interaction between circularly permuted green fluorescent protein and trypsin by total internal reflection fluorescence microscopy
چکیده انگلیسی
We have applied total internal reflection fluorescence microscopy (TIRFM) to visualize the dynamic interaction between circularly permuted green fluorescent protein (cpGFP) and trypsin at the single-molecule level. When trypsin was added, the fluorescence intensities of cpGFP mutants immobilized on the surface decreased dramatically with time. We also found that such decrease depended on the structural stability of the mutants. Furthermore, the phenomenon of 'on-off' blinking was observed in both the absence and presence of trypsin. Statistical analysis showed that the on-time decreased as reaction time increased.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Volumes 284–285, 15 August 2006, Pages 395-400
نویسندگان
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