Cationic vesicles based on non-ionic surfactant and synthetic aminolipids mediate delivery of antisense oligonucleotides into mammalian cells

- A synthetic aminolipid and non-ionic surfactant polysorbate-80 are able to condense with an antisense oligonucleotide forming cationic vesicles lipoplexes.
- Lipoplexes were characterized in terms of morphology, Zeta potential, electrophoretic mobility shift assay and dynamic light scattering.
- Lipoplexes at their optimal N/P ratio did not induce cytotoxic effects.
- Lipoplexes at their optimal N/P ratio mediated cellular uptake in mammalian cells obtaining good inhibition efficiencies in antisense therapy.