کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
599851 | 1454293 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A sepiolite modified conducting polymer based biosensor A sepiolite modified conducting polymer based biosensor](/preview/png/599851.png)
• A conducting polymer modified with sepiolite biosensor for successful biosensor applications.
• Adsorption technique for beneficial enzyme immobilization.
• Sensitive, reliable and long-life cholesterol biosensor.
• Successful application for the determination of cholesterol in food samples.
A conducting polymer modified with sepiolite was utilized in the construction of a highly sensitive and fast amperometric cholesterol biosensor. In this study a monomer; (10,13-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)dibenzo[a,c ]phenazine (PHED)) was synthesized and then its polymer was coated on a graphite electrode by electropolymerization to obtain a matrix for enzyme immobilization. Cholesterol oxidase was immobilized onto polymer coated electrode by adsorption technique. Sepiolite was introduced for a successful immobilization of the cholesterol oxidase. Immobilized enzyme kinetic parameters (KMapp, Imax) were evaluated by Michaelis-Menten kinetics and calculated as 0.031 mM and 6.06 μA, respectively. LOD and sensitivity were estimated as 0.36 μM and 1.64 mA/mMcm2. Characterization of designed biosensor was done to examine the effect of various factors such as enzyme amount, optimum pH and shelf-life. A novel accurate and inexpensive cholesterol biosensor was developed for the determination of total cholesterol in food samples.
Figure optionsDownload as PowerPoint slide
Journal: Colloids and Surfaces B: Biointerfaces - Volume 111, 1 November 2013, Pages 549–555