کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
600784 1454313 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence behavior of non-functionalized carbon nanoparticles and their in vitro applications in imaging and cytotoxic analysis of cancer cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Fluorescence behavior of non-functionalized carbon nanoparticles and their in vitro applications in imaging and cytotoxic analysis of cancer cells
چکیده انگلیسی

We report fluorescence behavior in non-functionalized carbon nanoparticles (NCNP) prepared from lamp soot and their application in imaging of normal and cancer cells. Structural characterization of these particles by Raman spectroscopy showed characteristic peaks located at 1350 and 1590 cm−1 corresponding to the diamond-like (D) and graphite-like (G) bands of the carbon allotropes respectively with the characteristic ratio ID/IG = 2.24. X-ray diffraction study confirmed the presence of amorphous as well as graphitized carbon in these nanostructures with minimum grain size ≈2 nm. A typical luminescence lifetime measured by time resolved fluorescence spectroscopy was obtained 3.54 ns. The photoluminescence behavior of these particles was excitation dependent and gave off blue, green and red fluorescence under UV, blue and green excitation, respectively. Cellular uptake of these NCNP yielded excellent results for cell imaging of human embryonic kidney, lung carcinoma and breast adenocarcinoma cells. Cell imaging was further correlated with cytotoxicity in the above mentioned cell lines and also in leukemia cell lines. Dose dependant cytotoxicity was observed after 24 h up to 48 h of incubation of nanoparticles. Fluorescence microscopy of nanoparticle-cell interaction clearly indicated aggregation of the particles.

Figure optionsDownload as PowerPoint slideHighlights
► Substrate effects on synthesis of carbon nanoparticles studied.
► Fluorescence in non-functionalized carbon nanoparticles observed.
► Cell imaging of normal and cancer cells using these particles demonstrated.
► Cytotoxicity and cell uptake of carbon nanoparticles probed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 91, 1 March 2012, Pages 34–40
نویسندگان
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