The anticonvulsant and sedative effects of Gladiolus dalenii extracts in mice

- Gladiolus dalenii possesses anticonvulsant activity.
- Gladiolus dalenii possesses sedative activity.
- Part of the anticonvulsant effects was antagonized by flumazenil and FG7142.
- Part of its effects is mediated through the benzodiazepine site receptor.

Gladiolus dalenii Van Geel is a medicinal plant commonly used in traditional medicine in Africa to treat epilepsy and many other diseases. Two in vivo mouse models (maximal electroshock and pentylenetetrazol-induced convulsions) were used to evaluate the anticonvulsant activities of the plant extracts. Diazepam-induced sleep was used for the evaluation of the sedative properties. The macerated extract of G. dalenii protected 100 and 83.3% of mice against PTZ- and MES-induced seizures, respectively. The aqueous extract of G. dalenii protected 100 and 83.3% of mice against PTZ- and MES-induced seizures, respectively. The lyophilized extract of G. dalenii also protected 100 and 83.3% of mice against PTZ- and MES-induced seizures, respectively. The coadministration of G. dalenii with diazepam resulted in an additive effect, while the coadministration of G. dalenii with flumazenil or FG7142 resulted in antagonistic effects. The macerate of G. dalenii also exerted sedative activity by reducing the latency time to sleep and increasing the total duration of sleep induced by diazepam. The sleeping time increased from 16 ± 3 min in the control group to 118 ± 11 min at a dose of 150 mg/kg of G. dalenii. The effects of G. dalenii suggested the presence of anticonvulsant and sedative activities that might show efficacy against secondarily generalized tonic-clonic seizures and primary generalized seizures and insomnia in humans.