کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
601350 879939 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA
چکیده انگلیسی

A nano-material carboxylic acid functionalized graphene (graphene-COOH) was prepared and used to construct a novel biosensor for the simultaneous detection of adenine and guanine. The direct electrooxidation behaviors of adenine and guanine on the graphene-COOH modified glassy carbon electrode (graphene-COOH/GCE) were carefully investigated by cyclic voltammetry and differential pulse voltammetry. The results indicated that both adenine and guanine showed the increase of the oxidation peak currents with the negative shift of the oxidation peak potentials in contrast to that on the bare glassy carbon electrode. The electrochemical parameters of adenine and guanine on the graphene-COOH/GCE were calculated and a simple and reliable electroanalytical method was developed for the detection of adenine and guanine, respectively. The modified electrode exhibited good behaviors in the simultaneous detection of adenine and guanine with the peak separation as 0.334 V. The detection limit for individual determination of guanine and adenine was 5.0 × 10−8 M and 2.5 × 10−8 M (S/N = 3), respectively. Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G + C)/(A + T) of single-stranded DNA was calculated as 0.80. The biosensor exhibited some advantages, such as simplicity, rapidity, high sensitivity, good reproducibility and long-term stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 82, Issue 2, 1 February 2011, Pages 543–549
نویسندگان
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