کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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601392 | 879941 | 2010 | 8 صفحه PDF | دانلود رایگان |
Polyamidoamine (PAMAM) dendrimers were covalently tethered onto the surface of multi-walled carbon nanotubes (MWCNTs). The morphology and dispersive properties of the MWCNT-PAMAM hybrids were characterized and the peripheral functional groups were identified. Cytotoxicity to human osteosarcoma MG-63 cells, and protein and DNA immobilization ability of the hybrids were evaluated in detail. The dendrimers were present on the surface of MWCNTs in high density. The MWCNT-PAMAM hybrids exhibited good dispersibility and stability in aqueous solution. We showed that the hybrids are biocompatible, with no obvious cytotoxicity at concentration <25 μg mL−1 over 24 h. They possess excellent biomolecule-immobilization ability (bovine serum albumin and 5′-Fam-CAAggTCgTgTAAAggTCAg-3′ were used as models). The functionalization of MWCNTs with PAMAM dendrimers improved the biomolecule-immobilization ability 70-fold and simultaneously decreased the cellular toxicity by about 30%. It is expected that the MWCNT-PAMAM hybrids will find promising applications in biosensors and biomolecule delivery systems in gene or protein therapy.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 80, Issue 1, 1 October 2010, Pages 18–25