کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6017436 1580167 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regular ArticleExperimental optic neuritis induced by the microinjection of lipopolysaccharide into the optic nerve
ترجمه فارسی عنوان
عصب بینایی منظم مقاله تجربی که توسط میکروکنترل لیپوپلی ساکارید به عصب بینایی منجر می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
چکیده انگلیسی


- We injected bacterial lipopolysaccharide (LPS) directly into the optic nerve.
- LPS induced a persistent decrease in visual evoked potentials and pupil reflex.
- LPS induced an early microglia/macrophage reactivity.
- LPS induced astrocytosis, demyelination, and axon and retinal ganglion cell loss.
- The injection of LPS could be a new experimental model of primary optic neuritis.

Optic neuritis (ON) is a condition involving primary inflammation, demyelination, and axonal injury in the optic nerve which leads to retinal ganglion cell (RGC) loss, and visual dysfunction. We investigated the ability of a single microinjection of bacterial lipopolysaccharide (LPS) directly into the optic nerve to induce functional and structural alterations compatible with ON. For this purpose, optic nerves from male Wistar rats remained intact or were injected with vehicle or LPS. The effect of LPS was evaluated at several time points post-injection in terms of: i) visual pathway and retinal function (visual evoked potentials (VEPs) and electroretinograms, (ERGs), respectively), ii) anterograde transport from the retina to its projection areas, iii) consensual pupil light reflex (PLR), iv) optic nerve histology, v) microglia/macrophage reactivity (by Iba-1- and ED1-immunostaining), vi) astrocyte reactivity (by glial fibrillary acid protein-immunostaining), vii) axon number (by toluidine blue staining), vii) demyelination (by myelin basic protein immunoreactivity and luxol fast blue staining), viii) optic nerve ultrastructure, and ix) RGC number (by Brn3a immunoreactivity). LPS induced a significant and persistent decrease in VEP amplitude and PLR, without changes in the ERG. In addition, LPS induced a deficit in anterograde transport, and an early inflammatory response consisting in an increased cellularity, and Iba-1 and ED1-immunoreactivity in the optic nerve, which were followed by changes in axonal density, astrocytosis, demyelination, and axon and RGC loss. These results suggest that the microinjection of LPS into the optic nerve may serve as a new experimental model of primary ON.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 266, April 2015, Pages 30-41
نویسندگان
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