Electrophoresis of human brain microvascular endothelial cells with uptake of cationic solid lipid nanoparticles: Effect of surfactant composition

This study analyzes the effects of Tween 80 and Span 20 on the electrical interaction between cationic solid lipid nanoparticles (CSLNs) and human brain microvascular endothelial cells (HBMECs). Electrophoretic mobility, zeta potential and fixed charge density of CSLNs and HBMECs with uptake of CSLNs were also investigated. The results revealed that a higher molar ratio of Tween 80 yielded smaller CSLNs, higher charge of CSLNs and larger electrical attraction energy between CSLNs and HBMECs. On the contrary, a decrease in the molar ratio of Tween 80 enhanced the absolute values of electrophoretic mobility and zeta potential of CSLNs-incorporating HBMECs. In addition, a higher concentration of CSLNs in the dispersion medium led to lower charge of CSLNs-incorporating HBMECs. An increased concentration of glutamate from 1 to 2 mg/mL enhanced the absolute values of electrophoretic properties of CSLNs-incorporating HBMECs. In the case of increasing concentration of glutamate, pure Tween 80 was stronger in charge enhancement than pure Span 20.