کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6021400 1580632 2016 39 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular analysis of acute and chronic reactive astrocytes in the pilocarpine model of temporal lobe epilepsy
ترجمه فارسی عنوان
تجزیه و تحلیل مولکولی آستروسیت های واکنش دهنده حاد و مزمن در مدل پیلوکارپین صرع لوب تمپورال
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
چکیده انگلیسی
Astroglia, the most abundant glial cells in the mammalian central nervous system (CNS), are considered an emerging key player in seizure induction and progression. Although astrocytes undergo reactive gliosis in temporal lobe epilepsy (TLE) with dramatic morphological and molecular changes, specific astrocyte targets/molecular pathways that contribute to the induction and progression of seizure remain largely unknown. By combining translating ribosomal affinity purification (TRAP) with the pilocarpine model of TLE in BAC aldh1l1 TRAP mice, we profiled translating mRNAs from hippocampal or cortical astrocytes at different phases (3 days, 30 days, and 60 days post-pilocarpine injections) of pilocarpine-induced epilepsy models. Our results found that hippocampal (but not cortical) astrocytes undergo early and unique molecular changes at 3 days post-pilocarpine injections. These changes indicate a potentially primary pathogenic role of hippocampal astrocytes in seizure induction and progression and provide new insights about the involvement of specific astrocytic pathways/targets in epilepsy. In particular, we validated expression changes of ocrl and aeg1 in pilocarpine models. Follow-up studies on these genes may reveal new roles of hippocampal astrocytes in TLE.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 91, July 2016, Pages 315-325
نویسندگان
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