Reduction of TRPV1 expression in the trigeminal system by botulinum neurotoxin type-A

► The effect of BoNT-A on TRPV1 expression was investigated in the trigeminal system. ► BoNT-A decreased TRPV1 expression in the trigeminal ganglion. ► BoNT-A inhibited TRPV1 trafficking to the plasma membrane. ► Cytoplasmic TRPV1 was predisposed to proteasome-mediated proteolysis. ► These data explain how BoNT-A relieves TRPV1-mediated pain.