کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6022609 1580683 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GABAergic signaling and connectivity on Purkinje cells are impaired in experimental autoimmune encephalomyelitis
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
GABAergic signaling and connectivity on Purkinje cells are impaired in experimental autoimmune encephalomyelitis
چکیده انگلیسی

A significant proportion of multiple sclerosis (MS) patients have functionally relevant cerebellar deficits, which significantly contribute to disability. Although clinical and experimental studies have been conducted to understand the pathophysiology of cerebellar dysfunction in MS, no electrophysiological and morphological studies have investigated potential alterations of synaptic connections of cerebellar Purkinje cells (PC). For this reason we analyzed cerebellar PC GABAergic connectivity in mice with MOG(35-55)-induced experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. We observed a strong reduction in the frequency of the spontaneous inhibitory post-synaptic currents (IPSCs) recorded from PCs during the symptomatic phase of the disease, and in presence of prominent microglia activation not only in the white matter (WM) but also in the molecular layer (ML). The massive GABAergic innervation on PCs from basket and stellate cells was reduced and associated to a decrease of the number of these inhibitory interneurons. On the contrary no significant loss of the PCs could be detected. Incubation of interleukin-1beta (IL-1β) was sufficient to mimic the electrophysiological alterations observed in EAE mice.We thus suggest that microglia and pro-inflammatory cytokines, together with a degeneration of basket and stellate cells and their synaptic terminals, contribute to impair GABAergic transmission on PCs during EAE. Our results support a growing body of evidence that GABAergic signaling is compromised in EAE and in MS, and show a selective susceptibility to neuronal and synaptic degeneration of cerebellar inhibitory interneurons.

► Cerebellar deficits are very frequent in patients with multiple sclerosis (MS). ► Inhibitory synaptic inputs to Purkinje cells show neurophysiological and pathological deficits in EAE, a mouse model of MS. ► The pro-inflammatory cytokine IL-1β also inhibits GABAergic transmission in acute cerebellar slice. ► Cerebellar GABAergic interneurons selectively degenerate in EAE in response to inflammation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 46, Issue 2, May 2012, Pages 414-424
نویسندگان
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