کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
602372 1454317 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and cell activity of novel galactosylated chitosan as a gene carrier
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Synthesis and cell activity of novel galactosylated chitosan as a gene carrier
چکیده انگلیسی

It is important for gene carrier to transport DNA into target cells. Although viral vectors are very efficient gene-transfer vehicles, significant drawbacks limit their applications. Chitosan (CS) has been researched widely as a non-viral vector. However, the low cell specificity and low transfection efficiency of chitosan need to be overcome. In order to conquer the drawback of chitosan, the present paper is concerned with the synthesis of novel galactosylated chitosan (GC) through etherization of chitosan and galactose in THF using BF3·OEt2 as promoter. The final product was characterized and confirmed by FT-IR and 1H NMR. The degree of O-substitution (DS) of chitosan by galactose was measured to be 10.38% using anthrone-sulfuric acid colorimetric method. The mean particle diameter and average zeta potential of the GC/DNA complex were 350 nm and +22.1 mV, respectively. The GC/DNA nanoparticle was tested to transfect HEK293 cells, and the viability of HEK293 cells was not affected by the GC/DNA nanoparticle compared to that of the control.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 70, Issue 2, 1 May 2009, Pages 181–186
نویسندگان
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