کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6115623 | 1215914 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multi-epitope proteins for improved serological detection of Trypanosoma cruzi infection and Chagas Disease
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کلمات کلیدی
T. cruziNECRDTIIFrapid diagnostic tests - آزمایش های تشخیص سریعantibodies - آنتی بادی هاIndirect immunofluorescence - ایمونوفلورسانس غیر مستقیمELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSerological diagnosis - تشخیص سلوکTandem repeat - تکرار دوبارهChagas disease - شاگاسAntigen - پادگِن یا آنتیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Multi-epitope proteins for improved serological detection of Trypanosoma cruzi infection and Chagas Disease Multi-epitope proteins for improved serological detection of Trypanosoma cruzi infection and Chagas Disease](/preview/png/6115623.png)
چکیده انگلیسی
We previously reported that tandem repeat (TR) proteins from Trypanosoma cruzi could serve as targets of the antibody response and be useful as diagnostic indicators. To optimize reagents for detecting T. cruzi infection we evaluated individual TR proteins and identified several that were recognized by the majority of Chagas patient's sera collected from individuals form Brazil. We then produced novel, recombinant fusion proteins to combine the reactive TR proteins into a single diagnostic product. Direct comparison of the antibody response of serum samples that were readily detected by the established fusion antigen used in commercial detection of Chagas disease, TcF, revealed strong responses to TcF43 and TcF26 proteins. While the TcF43 and TcF26 antigens enhanced detection and strength of signal, they did not compromise the specificity of detection compared to that obtained with TcF. Finally, it was apparent by testing against a panel of 84 serum samples assembled on the basis of moderate or weak reactivity against TcF (mostly signal:noise <5) that TcF43 and TcF26 could more strongly detected by many of the sera that had low TcF antibody levels. Taken together, these data indicate that TcF43 and TcF26 could be used to enhance the detection of T. cruzi infection as well as supporting a diagnosis of Chagas disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 84, Issue 3, March 2016, Pages 191-196
Journal: Diagnostic Microbiology and Infectious Disease - Volume 84, Issue 3, March 2016, Pages 191-196
نویسندگان
Malcolm S. Duthie, Jeffery A. Guderian, Aarthy C. Vallur, Ayesha Misquith, Hong Liang, Raodoh Mohamath, Alejandro O. Luquetti, Darrick Carter, Suelene N.B. Tavares, Steven G. Reed,