کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6115669 1591336 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Serotyping of pneumococci: evaluation of the genetic approach and performance with clinical samples
ترجمه فارسی عنوان
سروتایپ پنوموکوک: ارزیابی رویکرد ژنتیکی و عملکرد با نمونه های بالینی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی
Determination of pneumococcal serotypes depends on a successful culture and the Quellung's reaction. However, in 2006, the capsular sequences of 90 different pneumococcal capsular loci were published, thus making “genetic” serotyping via PCR possible. We wanted to determine the reliability of the published primers for the 13 serotypes included in pneumococcal conjugated vaccine 13 (PCV13) with pneumococcal isolates from Germany. We used a multiplex PCR approach and agarose gel detection of amplicons. Three hundred ninety well-characterized strains of Streptococcus pneumoniae and 46 clinical samples were used in the study. A 100% concordance was achieved between PCR and Quellung's reaction. In 7 clinical samples with a PCR positive for S. pneumoniae, we could determine a serotype included in PCV13.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 80, Issue 4, December 2014, Pages 274-277
نویسندگان
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