کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6116714 | 1216379 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intestine-derived Clostridium leptum induces murine tolerogenic dendritic cells and regulatory T cells in vitro
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کلمات کلیدی
BMDCsFCSOVAIBDqRT-PCRDCsGAPDHAPCsGM-CSFTregsmAbFOXP3MTCLPSgranulocyte-macrophage colony stimulating factorFACSPBSiDCssDCs3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromidePMA - LDC هاMTT - MTTMonoclonal antibody - آنتی بادی مونوکلونالantigen-presenting cells - آنتیژن ارائه سلولstandard deviation - انحراف معیارOvalbumin - اوبلبومینinterleukin - اینترلوکینInflammatory bowel disease - بیماریهای التهابی رودهEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاforkhead box P3 - جعبه جعبه P3fluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسfetal calf serum - سرم گوساله جنینDendritic cells - سلول های دندریتیکimmature dendritic cells - سلولهای دندریتیک نابالغRegulatory T cells - سلولهای تی تنظیمکنندهlipopolysaccharide - لیپوپلی ساکاریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریGlyceraldehyde phosphate dehydrogenase - گلیسرالیدید فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Intestine-derived Clostridium leptum induces murine tolerogenic dendritic cells and regulatory T cells in vitro Intestine-derived Clostridium leptum induces murine tolerogenic dendritic cells and regulatory T cells in vitro](/preview/png/6116714.png)
چکیده انگلیسی
Patients with autoimmune and allergic diseases frequently present with reduced numbers and functionally impaired regulatory T cells (Tregs) and/or tolerogenic dendritic cells (tDCs). tDC-mediated regulation of Treg proliferation (numbers) and activation is crucial to establishing and maintaining an appropriate level of immune tolerance. Colonic colonization of Clostridium spp. is associated with accumulation of Tregs, which inhibits development of inflammatory lesions. To investigate whether infection with the Clostridium leptum sp. can specifically induce Tregs and/or tDCs bone marrow-derived dendritic cells were cultured in the presence or absence of C. leptum then co-cultured with CD4+CD25â T cells or not. Changes in tDC numbers, Treg numbers, percentages of T cell subsets, and expression of cytokines related to Tregs (IL-10 and transforming growth factor-beta (TGF-β1)), DCs (IL-12p40 and IL-6) and effector T cells (IFN-γ, IL-4, IL-5, IL-13, and IL-17A) were measured. In the co-culture system, C. leptum-stimulated tDCs were able to increase the percentage and total number of Tregs attenuate activation of T helper cells (Th1, Th2, and Th17), and decrease the amount of secreted IL-4, IL-5, IL-13, IFN-γ and IL-17A. Thus, C. leptum exposure can induce the tDC-mediated stimulation of Tregs while disrupting the immune inflammatory response mediated by Th1, Th2 and Th17 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 75, Issue 12, December 2014, Pages 1232-1238
Journal: Human Immunology - Volume 75, Issue 12, December 2014, Pages 1232-1238
نویسندگان
Ya-Nan Li, Fei Huang, Huan-Ji Cheng, Shan-Yu Li, Li Liu, Li-Ying Wang,