کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6116839 | 1216395 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inter and intra laboratory concordance of HLA antibody results obtained by single antigen bead based assay
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کلمات کلیدی
MFICDCAMRSABDSAdonor specific antibody - آنتی بادی اختصاصی اهدا کنندهantibody mediated rejection - آنتی بادی مهار شده ردstandard deviation - انحراف معیارCoefficient of Variation - ضریب تغییرcomplement dependent cytotoxicity - مکمل سمیت مرکزی وابسته استmean fluorescence intensity - میانگین شدت فلورسانسPositive control - کنترل مثبتnegative control - کنترل منفی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Single antigen bead based assays (SAB) to identify antibodies to HLA are marketed as a qualitative test, however often used as a quantitative test as the results provide mean fluorescence intensity (MFI) which is found to correlate with the strength/avidity of the antibody. We studied the between and within laboratory variability in performing the SAB from one manufacturer. Ten samples were tested at four laboratories according to the manufacturer's suggested protocol. Additionally same samples were tested on four consecutive days in one laboratory. All tests were performed using the same lot of beads and secondary antibody. Results were classified as positive at four different MFI cutoffs: 1000, 5000, 8000 and 10,000. MFI values across and within the laboratory were compared in a pair-wise fashion with Pearson's correlations. Overall concordance for Class-I was 97% between laboratories and 98% within laboratory at all cutoffs. Pair-wise Pearson correlation between laboratories was 0.989-0.99, while within laboratory it was 0.998-0.999. For Class-II, overall concordance between and within laboratory was 98%. Pair-wise Pearson correlation between laboratories was 0.991-0.997, while within laboratory it was 0.997-0.999. There is good correlation between laboratories and within laboratory using the same manufacturer, same lot and same protocol while performing SAB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 74, Issue 3, March 2013, Pages 310-317
Journal: Human Immunology - Volume 74, Issue 3, March 2013, Pages 310-317
نویسندگان
Manish J. Gandhi, Steven DeGoey, Deborah Falbo, Sarah Jenkins, James R. Stubbs, Harriet Noreen, David F. Lorentzen, JarHow Lee, Mark Stegall,