کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6122915 | 1219612 | 2015 | 10 صفحه PDF | دانلود رایگان |
- Immunisation programmes against MenB are now a possibility.
- We describe meningococcal carriage rates in UK adolescents to inform strategy.
- Laboratory methods of detection and serogrouping are compared.
- Acquisition rate is estimated from longitudinal sampling data.
- The majority of MenB isolates had fHBP variants from subfamily A.
SummaryObjectivesRecent development of serogroup B meningococcal (MenB) vaccines highlights the importance of pharyngeal carriage data, particularly in adolescents and young adults, to inform implementation strategies. We describe current UK carriage prevalence in this high risk population and compare methods of carriage detection.MethodsIn this multisite study, pharyngeal swabs were collected on 3-4 occasions over 6-12 months, from 1040 school and university students, aged 10-25 years. Meningococcal carriage was detected by standard culture combined with seroagglutination or PCR of cultured isolates, or by direct PCR from swab. The factor H binding protein (fHBP) variants present in meningococcal isolates were determined.ResultsMeningococcal serogroups B and Y were most common, with carriage up to 6.5% and 5.5% respectively, increasing throughout adolescence. Identification by seroagglutination was often unreliable, and the sensitivity of direct PCR detection was 66% compared to culture combined with PCR. Of MenB isolates, 89.1% had subfamily A variants of fHBP. The acquisition rate of MenB carriage was estimated at 2.8 per 1000 person-months.ConclusionsIf vaccination is to precede the adolescent rise in MenB carriage, these data suggest it should take place in early adolescence. Studies assessing vaccine impact should use molecular methods to detect carriage.
Journal: Journal of Infection - Volume 71, Issue 1, July 2015, Pages 43-52