An improved DNA isolation technique for PCR detection of Strongyloides stercoralis in stool samples

► DNA yield and purity improved with the IH method. ► IH method includes incubation of stool samples in glycine-SDS buffer plus mechanical disruption. ► S. stercoralis was detected in all patients by the IH method but only in 9/17 by APC in the first sample. ► The higher efficiency of the IH method is demonstrated in clinical samples with low parasitic burden.