Production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of Fasciola gigantica

► The Ig isotype of the MoAb was found to be IgG1 and κ light chain isotype. ► FgSAP-2 is present at high level in WB and ES but not tegumental antigen (TA). ► This MoAb is specifically reacted with FgSAP-2, not with other parasites. ► FgSAP-2 was localized to the cytoplasm of caecal epithelial cells of late stages. ► This MoAb could be used for immunodiagnosis of ruminant and human fasciolosis.