Geographical distribution updating of Tunisian leishmaniasis foci: About the isoenzymatic analysis of 694 strains

► Isoenzymatic identification of 694 Leishmania strain isolated between 1998 and 2007 from Tunisian foci. ► L. infantum is the single species responsible for VL and MON-1 has a wide distribution. ► L. infantum, L. major and L. killicki are responsible for cutaneous leishmaniasis. ► Dermotropic L. infantum species has spread to the Centre and South part of the country. ► Leishmania killicki distribution is no longer restricted to the Southeast Tunisia.