Lack of protective efficacy in buffaloes vaccinated with Fasciola gigantica leucine aminopeptidase and peroxiredoxin recombinant proteins

► Leucine aminopeptidase gene was identified in Fasciola gigantica. ► Recombinant leucine aminopeptidase did not evoke protective response in buffaloes. ► Peroxiredoxin recombinant protein in combination with leucine aminopeptidase also failed to evoke protection in buffaloes.