کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6128434 | 1592802 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Species-specific PCR assay for L. infantum/L. donovani discrimination
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Species-specific PCR assay for L. infantum/L. donovani discrimination Species-specific PCR assay for L. infantum/L. donovani discrimination](/preview/png/6128434.png)
چکیده انگلیسی
Leishmania infantum and Leishmania donovani both pertain to the L. (L.) donovani complex. The status of certain strains is questioned in the literature and there are no reliable discriminative markers to identify them. Molecular tools are needed to (i) identify diagnostic markers and (ii) to allow a better understanding of phylogenetic relationships. We have developed a PCR based on cysteine protease B (cpb). This PCR discriminates between L. infantum and L. donovani with 50-100Â pg of DNA. These two species are differentiated by their fragment length. Indeed, L. donovani strains were characterized by a 741-bp product and L. infantum strains by a 702-bp product, except for one strain, which revealed a heterozygous profile with the two products. This PCR does not generate amplification for other Leishmania or kinetoplastids and could contribute to clarify the phylogenetic status of several taxa that are also being debated, such as L. archibaldi.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 100, Issue 3, December 2006, Pages 241-245
Journal: Acta Tropica - Volume 100, Issue 3, December 2006, Pages 241-245
نویسندگان
Mallorie Hide, Anne-Laure Bañuls,