Metabolomic analysis of Entamoeba: applications and implications

Entamoeba histolytica is an enteric protozoan parasite that causes hemorrhagic dysentery and extraintestinal abscesses in millions of inhabitants of endemic areas. The genome of E. histolytica has already been sequenced and used to predict the metabolic potential of the organism. Since nearly 56% of the E. histolytica genes remain unannotated, correlative 'omics' analyses of genomics, transcriptomics, proteomics, and biochemical metabolic profiling are essential in uncovering new, or poorly understood metabolic pathways. Metabolomics aims at understanding biology by comprehensive metabolite profiling. In this review, we discuss recent metabolomics approaches to elucidate unidentified metabolic systems of this pathogen and also discuss future applications of metabolomics to understand the biology and pathogenesis of E. histolytica.