Form and function in the trypanosomal secretory pathway

► The trypanosome secretory pathway is streamlined for efficient transport. ► GPI anchors influence selective transport in both early and post-Golgi compartments. ► ER exit sites and associated Golgi nucleate on unique flagellar cytoskeletal elements. ► A novel bilobe structure plays multiple roles in cytoskeletal and organellar replication. ► Unusual pathways of biosynthetic trafficking to the lysosome exist.