کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
6132677 1593440 2016 4 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
A one-step centrifugal ultrafiltration method to concentrate enteric viruses from wastewater
ترجمه فارسی عنوان
یک روش اولترافیلتراسیون گریز از مرکز یک مرحله ای برای غلبه بر ویروس های انتروکوکسی از فاضلاب
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موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی
A one-step centrifugal ultrafiltration method was developed to enhance rapid detection of human enteric viruses and co-occurring viruses in wastewater. Samples were collected pre- and post-UV treatment at two full-scale tertiary municipal wastewater treatment plants in Calgary, Canada. Viruses were concentrated from 100 mL wastewater samples through direct centrifugation using the Centricon Plus-70 ultrafilter. Seven viruses, including norovirus, rotavirus, sapovirus, astrovirus, enterovirus, adenovirus and JC virus, were tested using real-time quantitative PCR (rt-qPCR) and cell culture. All of the viruses were detected in pre- and post-UV samples by rt-qPCR, with rotavirus the most numerous (6.6 log10 GE copies/L). Infectious viruses, by cell culture, were found in all tested pre-UV samples but only in one post-UV sample. The results were comparable and consistent to that obtained using virus adsorption-elution method, indicating that the centrifugal ultrafiltration method is adequate to retain the viruses and maintain their infectivity during processing. As a simple, rapid and cost-effective method to screen wastewater viruses, this one-step centrifugal ultrafiltration method may serve as an effective approach to assess virus removal and gain knowledge of human virus activity during wastewater treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 237, November 2016, Pages 150-153
نویسندگان
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