کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6132679 1593440 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Titration of individual strains in trivalent live-attenuated influenza vaccine without neutralization
ترجمه فارسی عنوان
تیتراسیون سویه های فردی در واکسن آنفولانزای مرغی باکتری زنده بدون خنثی سازی
کلمات کلیدی
واکسن آنفلوآنزا واجد شرایط زنده، ویروس آنفولانزا، تیتراسیون واکسن سه گانه،
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی
Formulation and quality control of trivalent live-attenuated influenza vaccine requires titration of infectivity of individual strains in the trivalent mix. This is usually performed by selective neutralization of two of the three strains and titration of the un-neutralized strain in cell culture or embryonated eggs. This procedure requires standard sera with high neutralizing titer against each of the three strains. Obtaining standard sera, which can specifically neutralize only the corresponding strain of influenza viruses and is able to completely neutralize high concentration of virus in the vaccine samples, can be a problem for many vaccine manufacturers as vaccine stocks usually have very high viral titers and complete neutralization may not be obtained. Here an alternative approach for titration of individual strain in trivalent vaccine without the selective neutralization is presented. This was done by detecting individual strains with specific antibodies in an end-point titration of a trivalent vaccine in cell culture. Similar titers were observed in monovalent and trivalent vaccines for influenza A H3N2 and influenza B strains, whereas the influenza A H1N1 strain did not grow well in cell culture. Viral interference among the vaccine strains was not observed. Therefore, providing that vaccine strains grow well in cell culture, this assay can reliably determine the potency of individual strains in trivalent live-attenuated influenza vaccines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 237, November 2016, Pages 154-158
نویسندگان
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