کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6132767 1593441 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
چکیده انگلیسی
A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 236, October 2016, Pages 215-220
نویسندگان
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