کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6132924 | 1593442 | 2016 | 6 صفحه PDF | دانلود رایگان |
- A 5-h protocol to purify dengue virus from contaminants, including C6/36 densovirus.
- Purification yields infective, intact virion.
- Pelleted virus can be re-suspended at desired concentration.
Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.
Journal: Journal of Virological Methods - Volume 235, September 2016, Pages 125-130