کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133105 | 1593454 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection
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کلمات کلیدی
AIVEID50RT-RPAIBVNDVHPAIRPART-LAMPILTcycle threshold - آستانه چرخهAvian influenza - آنفلوآنزای مرغیhighly pathogenic avian influenza - آنفلوآنزای پرندگان بسیار patogenezRecombinase polymerase amplification - تقویت پلیمراز RecombinaseReal-time RT-PCR - زمان واقعی RT-PCRInfectious laryngotracheitis - لارنگوتراشیت عفونیMycoplasma gallisepticum - مایکوپلاسما گالسیپتیکومNucleotides - نوکلئوتیدhemagglutinin - هماگلوتینینreal-time reverse transcription polymerase chain reaction - واکنش زنجیره پلیمراز رونویسی معکوس در زمان واقعیavian influenza virus - ویروس آنفولانزای مرغیInfectious bronchitis virus - ویروس برونشیت عفونیNewcastle disease virus - ویروس بیماری نیوکاسل
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The 2006 outbreaks of H5N1 avian influenza in Egypt interrupted poultry production and caused staggering economic damage. In addition, H5N1 avian influenza viruses represent a significant threat to public health. Therefore, the rapid detection of H5 viruses is very important in order to control the disease. In this study, a qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of hemagglutinin gene of H5 subtype influenza viruses was developed. The results were compared to the real-time reverse transcription polymerase chain reaction (RT-PCR). An in vitro transcribed RNA standard of 970 nucleotides of the hemagglutinin gene was developed and used to determine the assay sensitivity. The developed H5 RT-RPA assay was able to detect one RNA molecule within 7Â min, while in real-time RT-PCR, at least 90Â min was required. H5 RT-RPA assay did not detect nucleic acid extracted from H5 negative samples or from other pathogens producing respiratory manifestation in poultry. The clinical performance of the H5 RT-RPA assay was tested in 30 samples collected between 2014 and 2015; the sensitivity of H5 RT-RPA and real-time RT-PCR was 100%. In conclusion, H5 RT-RPA was faster than real-time RT-PCR and easily operable in a portable device. Moreover, it had an equivalent sensitivity and specificity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 223, October 2015, Pages 45-49
Journal: Journal of Virological Methods - Volume 223, October 2015, Pages 45-49
نویسندگان
Nahed Yehia, Abdel-Satar Arafa, Ahmed Abd El Wahed, Ahmed A. El-Sanousi, Manfred Weidmann, Mohamed A. Shalaby,