کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133395 | 1593462 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Degenerate PCR primer design for the specific identification of rhinovirus C
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کلمات کلیدی
HEVORFRT-PCRRhinovirus CMSAqRT-PCRN-termHuman rhinovirus CHRV - ENGamino acid - آمینو اسیدHuman enteroviruses - انتروویروس های انسانیMultiple sequence alignments - ترازهای توالی چندگانهamino terminal - ترمینال آمینHuman rhinovirus - رینوویروس انسانیopen reading frame - قاب خواندن بازUntranslated regions - مناطق غیر ترجمهUTR یا untranslated regions - منطقه ترجمه نشدهreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Degenerate PCR primer design for the specific identification of rhinovirus C Degenerate PCR primer design for the specific identification of rhinovirus C](/preview/png/6133395.png)
چکیده انگلیسی
Human rhinovirus (HRV)-A and -B is a common cause of upper respiratory tract infections. Recently, a third species, HRV-C, was categorized based on molecular typing studies. The results showed that the HRV-C genome had diverged from that of HRV-A and -B. Despite its late identification, increasing evidence suggests that HRV-C causes more severe pathogenic infections than HRV-A or -B; however, a large amount of epidemiological data is required to confirm this association in different clinical settings. Consequently, a simple and rapid method for identifying HRV-C is required to expedite such epidemiological studies. Here, two degenerate primer sets (HEV and HRVC) were designed based on bioinformatic analyses. The HEV set targeting the fifth IRES domain sequence within the 5â²-UTR, which is highly conserved among enteroviruses, was designed to detect all enteroviruses, whereas the HRVC set, which targeted the VP2 coding region, was designed to detect HRV-C alone. Both primer sets were tested against a panel of standard enteroviruses and clinical lavage samples. HEV detected all enteroviruses tested whereas HRVC was specific for HRV-C. Although the primer design strategy was confirmed with a limited number of samples, extensive tests are required to be applied in clinical settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 214, 15 March 2015, Pages 15-24
Journal: Journal of Virological Methods - Volume 214, 15 March 2015, Pages 15-24
نویسندگان
Young Ran Nam, Uk Lee, Han Seok Choi, Kyoung Jin Lee, Nari Kim, Yong Ju Jang, Chul Hyun Joo,