An improved validated SYBR green-based real-time quantitative PCR assay for the detection of the Penaeus stylirostris densovirus in penaeid shrimp

- A SYBR-Green based real-time PCR for IHHNV detection was developed.
- Current diagnostic PCR tests may result in false-positive results.
- This assay is highly sensitive, specific, economical, reproducible, and accurate.