کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6133524 1593468 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationDevelopment of a novel protocol for generating flavivirus reporter particles
ترجمه فارسی عنوان
ارتباط کوتاه توسعه یک پروتکل جدید برای تولید ذرات خبرنگار فلاویویروس
کلمات کلیدی
ویروس نیل غربی، ذرات ویروس گزارشگر، پروتکل،
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- WNV replicon RNA without any antibiotic genes was transfected into BHK cells.
- The replicon was maintained in the cells.
- RVPs could be rescued from the cells by expression of virus structural genes.

Infection with West Nile virus (WNV), a mosquito-borne flavivirus, is a growing public and animal health concern worldwide. Prevention, diagnosis and treatment strategies for the infection are urgently required. Recently, viral reverse genetic systems have been developed and applied to clinical WNV virology. We developed a protocol for generating reporter virus particles (RVPs) of WNV with the aim of overcoming two major problems associated with conventional protocols, the difficulty in generating RVPs due to the specific skills required for handling RNAs, and the potential for environmental contamination by antibiotic-resistant genes encoded within the genome RNA of the RVPs. By using the proposed protocol, cells were established in which the RVP genome RNA is replicated constitutively and does not encode any antibiotic-resistant genes, and used as the cell supply for RVP genome RNA. Generation of the WNV RVPs requires only the simple transfection of the expression vectors for the viral structural proteins into the cells. Therefore, no RNA handling is required in this protocol. The WNV RVP yield obtained using this protocol was similar that obtained using the conventional protocol. According to these results, the newly developed protocol appears to be a good alternative for the generation of WNV RVPs, particularly for clinical applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 208, November 2014, Pages 96-101
نویسندگان
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