کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133641 | 1593469 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrogen peroxide inactivation of influenza virus preserves antigenic structure and immunogenicity
ترجمه فارسی عنوان
غیرفعال کردن پراکسید هیدروژن ویروس آنفلوآنزا ساختار آنتی ژنیک و ایمنی زایی را حفظ می کند
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کلمات کلیدی
آب اکسیژنه، ویروس آنفولانزا، غیر فعال کردن ایمنی پاسخ ایمنی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
The use of live virus in the laboratory requires additional precautions, such as personnel training and special equipment, in order to limit the transmission risk. This is a requirement which not all laboratories can fulfill. In this study, a viral inactivation method is introduced using hydrogen peroxide (H2O2), which maintains antigenicity. Three strains of influenza viruses were inactivated and the ex vivo cellular and humoral immune responses were further analyzed, by comparing them to live viruses, in ELISpot, Multiplex and ELISA assays. In all assays, the H2O2 inactivated viruses displayed comparable responses to the live viruses, suggesting that the inactivated viruses still elicited immunogenic responses even though inactivation was confirmed by lack of viral replication in MDCK cells. Taken together, this study demonstrates that influenza viruses inactivated with H2O2 retain immunogenicity and are able to both detect humoral and elicit cellular immune responses in vitro, which could reduce the need to handle live viruses in the laboratory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 207, October 2014, Pages 232-237
Journal: Journal of Virological Methods - Volume 207, October 2014, Pages 232-237
نویسندگان
Jennifer L. Dembinski, Olav Hungnes, Anna Germundsson Hauge, Anne-Cathrine Kristoffersen, Bjørn Haneberg, Siri Mjaaland,