کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6133727 1593473 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel set of polyvalent primers that detect members of the genera Bromovirus and Cucumovirus
ترجمه فارسی عنوان
یک مجموعه رمان از پرایمرهای چند منظوره که اعضای خانواده های بروموی ویروس و کوکو ویروس را تشخیص می دهند
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی
Rapid detection and diagnosis of plant virus infection is one of the most important steps in preventing damages caused by viral diseases. Bromoviruses and cucumoviruses belong to the family Bromoviridae, which is one of the most important families of plant viruses, and infect a broad range of host plants including various economically important crops. In this study, an RT-PCR assay was developed for the universal detection of bromoviruses and cucumoviruses using a set of primers designed to target the conserved sequences in viral RNA1. The assay detected three species of Cucumovirus (Cucumber mosaic virus (CMV), Peanut stunt virus (PSV) and Tomato aspermy virus (TAV)) and two species of Bromovirus (Brome mosaic virus (BMV) and Cowpea chlorotic mottle virus (CCMV)) with high specificity and sensitivity. The assay developed in this study is predicted to have the potential to detect all major members of the genera Bromovirus and Cucumovirus and to be used as a routine diagnostic assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 203, July 2014, Pages 112-115
نویسندگان
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