کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6134111 | 1223578 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis](/preview/png/6134111.png)
چکیده انگلیسی
Foot-and-mouth disease (FMD) is a highly contagious epidemic disease threatening the cattle industry since the sixteenth century. In recent years, the development of diagnostic assays for FMD has benefited considerably from the advances of recombinant DNA technology. In this study, the immunodominant region of the capsid protein VP1 of the foot-and-mouth disease virus (FMDV) was fused to the T7 bacteriophage and expressed on the surface of the bacteriophage capsid protein. The recombinant protein of about 42Â kDa was detected by the anti-T7 tag monoclonal antibody in Western blot analysis. Phage ELISA showed that both the vaccinated and positive infected bovine sera reacted significantly with the recombinant T7 particle. This study demonstrated the potential of the T7 phage displaying the VP1 epitope as a diagnostic reagent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 193, Issue 2, November 2013, Pages 611-619
Journal: Journal of Virological Methods - Volume 193, Issue 2, November 2013, Pages 611-619
نویسندگان
Chuan Loo Wong, Chin Chin Sieo, Wen Siang Tan,