کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6134216 | 1593482 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment of reference gene stability influenced by extremely divergent disease symptoms in Solanum lycopersicum L.
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کلمات کلیدی
TBPRAN1Ribosomal protein L8RPL8TMV18S rRNAACTRT-qPCRCyPCACGAPDHEXPEF1α - EF1a18S ribosomal RNA - RNA ریبوزوم 18Sα-Tubulin - α-توبولینactin - اکتین quantitative reverse transcription-PCR - رونویسی معکوس PCRCMV - سیتومگالوویروسcyclophilin - سیکلوفیلینSand - شنelongation factor 1α - ضریب انحنای 1αtub - وانTobacco mosaic virus - ویروس موزائیک توتونCucumber mosaic virus - ویروس موزاییک خیارPepino mosaic virus - ویروس موزاییک پپینوPlant viruses - ویروس های گیاهیTATA-binding protein - پروتئین متصل به TATAReference genes - ژنهای مرجعglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازSolanum lycopersicum - گوجه فرنگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Parallel with the development of contemporary genetics and the constant increase in sequencing data, progress has to be aligned with improvement of experimental methods used for studying genes functions and gene expression levels, of which the quantitative polymerase chain reaction (qPCR) is still the most reliable. As well as with other nucleic acid-based methods used for comparison of the abundance of specific RNAs, the RT-qPCR data have to be normalised to the levels of RNAs represented stably in a cell. To achieve the goal, the so-called housekeeping genes (i.e., RNAs encoding, for instance, proteins playing an important role in the cell metabolism or structure maintenance), are used for normalisation of the target gene expression data. However, a number of studies have indicated the transcriptional instability of commonly used reference genes analysed in different situations or conditions; for instance, the origin of cells, tissue types, or environmental or other experimental conditions. The expression of ten common housekeeping genes of S. lycopersicum, namely EF1α, TUB, CAC, EXP, RPL8, GAPDH, TBP, ACT, SAND and 18S rRNA were examined during viral infections of tomato. Changes in the expression levels of the genes were estimated by comparison of the non-inoculated tomato plants with those infected with commonly known tomato viral pathogens, Tomato torrado virus, Cucumber mosaic virus, Tobacco mosaic virus and Pepino mosaic virus, inducing a diverse range of disease symptoms on the common host, ranging from mild leaves chlorosis to very severe stem necrosis. It is emphasised that despite the wide range of diverse disease symptoms it is concluded that ACT, CAC and EF1α could be used as the most suitable reference genes in studies of host-virus interactions in tomato.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 194, Issues 1â2, December 2013, Pages 161-168
Journal: Journal of Virological Methods - Volume 194, Issues 1â2, December 2013, Pages 161-168
نویسندگان
PrzemysÅaw Wieczorek, Barbara WrzesiÅska, Aleksandra ObrÄpalska-StÄplowska,