Calibrated real-time polymerase chain reaction for specific quantitation of HHV-6A and HHV-6B in clinical samples

► A novel molecular approach for the reliable quantification of both HHV-6A and B DNA load in different types of biological samples. ► The design ensures specific quantification of both viruses in case of mixed infections. ► Both assays are multiplexed with the calibrator system developed to control PCR artifacts.