Immunodetection of Triticum mosaic virus by DAS- and DAC-ELISA using antibodies produced against coat protein expressed in Escherichia coli: Potential for high-throughput diagnostic methods

► Bacterially expressed TriMV coat protein (CP) was purified as a soluble native form. ► Polyclonal antibodies produced against in vitro expressed TriMV CP in rabbits. ► Contrary to other viruses, TriMV antibodies reacted positively with native virions in DAS-ELISA. ► In DAS- and DAC-ELISA, antibodies specifically and sensitively detected TriMV in crude sap. ► Potential to develop high-throughput diagnostic methods for TriMV.