کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6134418 | 1223584 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunodetection of Triticum mosaic virus by DAS- and DAC-ELISA using antibodies produced against coat protein expressed in Escherichia coli: Potential for high-throughput diagnostic methods
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunodetection of Triticum mosaic virus by DAS- and DAC-ELISA using antibodies produced against coat protein expressed in Escherichia coli: Potential for high-throughput diagnostic methods Immunodetection of Triticum mosaic virus by DAS- and DAC-ELISA using antibodies produced against coat protein expressed in Escherichia coli: Potential for high-throughput diagnostic methods](/preview/png/6134418.png)
چکیده انگلیسی
⺠Bacterially expressed TriMV coat protein (CP) was purified as a soluble native form. ⺠Polyclonal antibodies produced against in vitro expressed TriMV CP in rabbits. ⺠Contrary to other viruses, TriMV antibodies reacted positively with native virions in DAS-ELISA. ⺠In DAS- and DAC-ELISA, antibodies specifically and sensitively detected TriMV in crude sap. ⺠Potential to develop high-throughput diagnostic methods for TriMV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 189, Issue 1, April 2013, Pages 196-203
Journal: Journal of Virological Methods - Volume 189, Issue 1, April 2013, Pages 196-203
نویسندگان
Satyanarayana Tatineni, Gautam Sarath, Dallas Seifers, Roy French,