Complete and repeatable inactivation of HIV-1 viral particles in suspension using a photo-labeled non-nucleoside reverse transcriptase inhibitor

► A simple methodology for achieving repeatable and complete inactivation of HIV. ► A photoactive PA-DAPYa can completely inactivate HIV reverse transcriptase. ► HIV can be inactivated by PA-DAPYa when irradiated with non-microbicidal UV light. ► Large scale inactivation of HIV viral particles in suspension for use in vaccination.