Simultaneous detection of porcine parvovirus and porcine circovirus type 2 by duplex real-time PCR and amplicon melting curve analysis using SYBR Green

► SYBR Green І duplex real-time PCR used to detect PPV and PCV-2. ► The distinctive melting temperature is different for PPV and PCV-2. ► The detection limit is 0.01 TCID50/mL for PPV and PCV-2.