Development and application of an indirect ELISA for detection of antibodies against avian hepatitis E virus

► We develop an iELISA to detect reactive antibodies against avian HEV genotype 3. ► The iELISA method had an agreement rate of 97% with Western blot analysis. ► Seropositivity of avian HEV genotype 3 is not age dependent. ► Antibodies titers peak is detected in 20-29 weeks old chickens.