Extraction and purification of hepatitis B virus-like M particles from a recombinant Saccharomyces cerevisiae strain using alumina powder

► Improvement of HBV M protein extraction by alumina powder from S. cerevisiae. ► Analysis by atomic force microscopy of the rVirus like particle. ► Purification of the rVLP by 2 steps of gel filtration: S-200 and SEC-250. ► Test of the immuno-reactivity of the rVLP on sera of individuals with hepatitis B.